-Assistant Professor, Pharmaceutical Chemistry and Chemical Engineering
-Biomolecular Engineering, Bioinformatics
- Post Doc Immunology & vaccinology, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, Bethesda, MD, 2017
- Ph.D. Biochemical Engineering, The University of Texas at Austin, Austin, TX, 2015
- B.S.. Chemical Engineering, The University of Kansas, Lawrence, KS, 2010
Next-generation DNA sequencing technologies have revolutionized the way that immunologists and biotechnologists study adaptive immune responses to vaccination and disease. The DeKosky laboratory works to invent and apply advanced NextGen molecular biology techniques to accelerate development of new human vaccines and therapeutics. We have a special focus on understanding highly effective human antibody responses to persistent viral infections (including HIV, EBV, CMV), and in characterizing the antibody response to experimental vaccines.
For more information please see our lab webpage, http://www.dekoskylab.org .
Keywords: Vaccines, Immunology, Virology, Molecular Biotechnology, Therapeutic Proteins, Antibody Discovery
Peer Reviewed Publications
Google Scholar link: https://scholar.google.com/citations?user=z6TwVlwAAAAJ&hl=en
DeKosky BJ, Kojima T, Rodin A, Charab W, Ippolito GC, Ellington AD, Georgiou G. In-depth determination and analysis of the human paired heavy and light chain antibody repertoire. Nature Medicine, 21, 86–91. 2014
McDaniel JR*, DeKosky BJ*, Tanno H, Ellington AD, Georgiou G, "Ultra-high throughput sequencing of the immune receptor repertoire from millions of lymphocytes," Nature Protocols. 11: 429-442. 2016
DeKosky BJ, Ippolito GC, Deschner RP, Lavinder JJ, Wine Y, Rawlings BM, Varadarajan N, Giesecke C, Dörner T, Andrews SF, Wilson PC, Hunicke-Smith SP, Willson CG, Ellington AD, Georgiou G. High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire. Nature Biotechnology. 31: 166-169. 2013
DeKosky, B. J., Lungu, O. I., Park, D., Johnson, E. L., Charab, W., Chrysostomou, C., Kuroda, D., Ellington, A. D., Ippolito, G. C., Gray, J. J. & Georgiou, G. Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires. Proceedings of the National Academy of Sciences. 113: E2636–E2645. 2016
Doria-Rose NA*, Schramm CA*, Gorman J*, Moore PL*, Bhiman JN, DeKosky BJ, Ernandes MJ, Georgiev IS, Kim HJ, Pancera M, Staupe RP, Altae-Tran HR, Bailer RT, Crooks ET, Cupo A, Druz A, Garrett NJ, Hoi KH, Kong R, Louder MK, Longo NS, McKee K, Nonyane M, O’Dell S, Roark RS, Rudicell RS, Schmidt SD, Sheward DJ, Soto C, Wibmer CK, Yang Y, Zhang Z, NISC Comparative Sequencing, Mullikin JC, Binley JM, Sanders RW, Wilson IA, Moore JP, Ward AB, Georgiou G, Williamson C, Abdool Karim SS, Morris L**, Kwong PD**, Shapiro L**, Mascola JR**. Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies. Nature 509: 55–62. 2014
Tian M*, Cheng C*, Chen X*, Duan H*, Cheng HL*, Dao M, Sheng Z, Kimble M, Wang L, Lin S, Schmidt SD, Du Z, Joyce MG, Chen Y, DeKosky BJ, Chen Y, Normandin E, Cantor E, Chen RE, Doria-Rose NA, Zhang Y, Shi W, Kong WP, Choe M, Henry AR, Laboune F, Georgiev IS, Huang PY, Jain S, McGuire AT, Georgeson E, Menis S, Douek DC, Schief WR, Stamatatos L, Kwong PD, Shapiro L, Haynes BF, Mascola JR & Alt FW. Induction of HIV neutralizing antibody lineages in mice with diverse precursor repertoires. Cell 166, 1471–1484.e18. 2016